Abstract
We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistant Staphylococcus aureus.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alberta / epidemiology
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Bacterial Toxins / genetics*
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Bacteriological Techniques
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Base Sequence
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Community-Acquired Infections / epidemiology
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Community-Acquired Infections / microbiology
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DNA, Bacterial / genetics
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Exotoxins / genetics*
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Genes, Bacterial*
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Genotype
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Humans
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Leukocidins
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Methicillin Resistance / genetics
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Phenotype
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / statistics & numerical data
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Staphylococcal Infections / epidemiology
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Staphylococcal Infections / microbiology
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / genetics*
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Staphylococcus aureus / pathogenicity
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Virulence / genetics
Substances
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Bacterial Toxins
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DNA, Bacterial
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Exotoxins
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Leukocidins
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Panton-Valentine leukocidin