Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci

J Clin Microbiol. 2006 Mar;44(3):1141-4. doi: 10.1128/JCM.44.3.1141-1144.2006.

Abstract

We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistant Staphylococcus aureus.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alberta / epidemiology
  • Bacterial Toxins / genetics*
  • Bacteriological Techniques
  • Base Sequence
  • Community-Acquired Infections / epidemiology
  • Community-Acquired Infections / microbiology
  • DNA, Bacterial / genetics
  • Exotoxins / genetics*
  • Genes, Bacterial*
  • Genotype
  • Humans
  • Leukocidins
  • Methicillin Resistance / genetics
  • Phenotype
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • Staphylococcal Infections / epidemiology
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / pathogenicity
  • Virulence / genetics

Substances

  • Bacterial Toxins
  • DNA, Bacterial
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin