Scatter factor mitigates HIV-1 gp120-induced human mesangial cell injury

Nephron Exp Nephrol. 2006;103(3):e103-8. doi: 10.1159/000092195. Epub 2006 Mar 22.

Abstract

HIV-1 gp120 protein has been shown to promote mesangial cell (MC) injury. Scatter factor (SF) is a growth factor that plays a reparative role in various experimental models of renal lesions. We hypothesize that SF protects MC against HIV-1 gp120-induced MC injury. gp120 at a low dose, stimulated HMC proliferation (p < 0.0001). SF (50 ng/ml) further enhanced low-dose gp120-induced HMC proliferation. However, gp120 at higher doses (10-100 ng/ml) promoted HMC apoptosis. Nevertheless, SF attenuated the high-dose gp120-induced HMC apoptosis. Interestingly, gp120 at a low dose not only induced NF-kappaB activation but also increased p21(cip1/waf1) andp27(kip1) protein levels.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Apoptosis / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Cyclin-Dependent Kinase Inhibitor p21 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p27 / metabolism
  • Dose-Response Relationship, Drug
  • HIV Envelope Protein gp120 / administration & dosage
  • HIV Envelope Protein gp120 / pharmacology*
  • Hepatocyte Growth Factor / pharmacology*
  • Humans
  • Mesangial Cells / drug effects*
  • Mesangial Cells / metabolism
  • Mesangial Cells / pathology*
  • Mesangial Cells / physiology
  • NF-kappa B / metabolism

Substances

  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Cyclin-Dependent Kinase Inhibitor p21
  • HIV Envelope Protein gp120
  • NF-kappa B
  • Cyclin-Dependent Kinase Inhibitor p27
  • Hepatocyte Growth Factor