Abstract
Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Viral / genetics*
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Disease Outbreaks
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Evolution, Molecular*
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Gene Deletion
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Genetic Variation*
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Genome, Viral*
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Genomics
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Humans
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Molecular Sequence Data
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Open Reading Frames
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Phylogeny
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Proteome / analysis
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Proteome / genetics
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Recombination, Genetic
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Sequence Analysis, DNA
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Smallpox / epidemiology
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Smallpox / mortality
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Smallpox / virology*
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Variola virus / classification
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Variola virus / genetics*
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Variola virus / isolation & purification
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Variola virus / pathogenicity
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Viral Proteins / chemistry
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Viral Proteins / genetics
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Virulence / genetics
Substances
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DNA, Viral
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Proteome
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Viral Proteins
Associated data
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GENBANK/DQ437580
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GENBANK/DQ437581
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GENBANK/DQ437582
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GENBANK/DQ437583
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GENBANK/DQ437584
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GENBANK/DQ437585
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GENBANK/DQ437586
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GENBANK/DQ437591
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GENBANK/DQ441416
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