In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs

G L Yu; J D Bradley; L D Attardi; E H Blackburn

doi:10.1038/344126a0

In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs

Nature. 1990 Mar 8;344(6262):126-32. doi: 10.1038/344126a0.

Authors

G L Yu¹, J D Bradley, L D Attardi, E H Blackburn

Affiliation

¹ Department of Molecular and Cell Biology, University of California, Berkeley.

PMID: 1689810
DOI: 10.1038/344126a0

Abstract

Mutating the CAACCCCAA sequence in the RNA component of telomerase causes the synthesis in vivo of new telomere sequences corresponding to the mutated RNA sequence, demonstrating that the telomerase contains the template for telomere synthesis. These mutations also lead to nuclear and cell division defects, and senescence, establishing an essential role for telomerase in vivo.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Base Sequence
DNA Nucleotidylexotransferase / metabolism*
Gene Expression
Genes
Genetic Vectors
Molecular Sequence Data
Mutation*
Plasmids
RNA / genetics*
Templates, Genetic
Tetrahymena / enzymology
Tetrahymena / genetics*

Substances

RNA
DNA Nucleotidylexotransferase