Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction

FEBS Lett. 1990 Apr 9;263(1):121-6. doi: 10.1016/0014-5793(90)80719-y.

Abstract

We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycemic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINm5F. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Hyperglycemia / genetics
  • Hyperglycemia / metabolism
  • Insulin / metabolism
  • Insulin Secretion
  • Insulinoma
  • Islets of Langerhans / metabolism
  • Islets of Langerhans / physiology*
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Mice
  • Mice, Obese
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Pancreatic Neoplasms
  • Poly A / genetics
  • Poly A / isolation & purification
  • Polymerase Chain Reaction
  • Potassium Channels / physiology*
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger

Substances

  • Insulin
  • Membrane Proteins
  • Oligonucleotide Probes
  • Potassium Channels
  • RNA, Messenger
  • Poly A
  • RNA