Complete genome sequence of the broad host range single-stranded RNA phage PRR1 places it in the Levivirus genus with characteristics shared with Alloleviviruses

Tanja M Ruokoranta; A Marika Grahn; Janne J Ravantti; Minna M Poranen; Dennis H Bamford

doi:10.1128/JVI.01005-06

Complete genome sequence of the broad host range single-stranded RNA phage PRR1 places it in the Levivirus genus with characteristics shared with Alloleviviruses

J Virol. 2006 Sep;80(18):9326-30. doi: 10.1128/JVI.01005-06.

Authors

Tanja M Ruokoranta¹, A Marika Grahn, Janne J Ravantti, Minna M Poranen, Dennis H Bamford

Affiliation

¹ Department of Biological and Environmental Sciences, Institute of Biotechnology, Viikki Biocenter 2, P.O. Box 56 (Viikinkaari 5), FIN-00014 University of Helsinki, Finland.

Abstract

Single-stranded RNA (ssRNA) bacteriophages of the family Leviviridae infect gram-negative bacteria. They are restricted to a single host genus. Phage PRR1 is an exception, having a broad host range due to the promiscuity of the receptor encoded by the IncP plasmid. Here we report the complete genome sequence of PRR1. Three proteins homologous with those of other ssRNA phages, i.e., maturation, coat, and replicase proteins, were identified. A fourth protein has a lysis function. Comparison of PRR1 with other members of the Leviviridae family places PRR1 in the genus Levivirus with some characteristics more similar to those of members of the genus Allolevivirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Allolevivirus / genetics*
Bacteriophages / metabolism
Base Sequence
Evolution, Molecular
Genome, Viral*
Levivirus / genetics*
Models, Genetic
Models, Molecular
Molecular Sequence Data
Nucleic Acid Conformation
Phylogeny
Plasmids / metabolism
RNA / genetics*

Substances

RNA

Associated data

GENBANK/DQ836063