Mouse transthyretin-related protein is a hydrolase which degrades 5-hydroxyisourate, the end product of the uricase reaction

Mol Cells. 2006 Oct 31;22(2):141-5.

Abstract

Uric acid is the end product of the purine degradation pathway in humans. It is catabolized to allantoin by urate oxidase or uricase (E.C. 1.7.3.3.) in most vertebrates except humans, some primates, birds, and certain species of reptiles. Here we provide evidence that mouse transthyretin-related protein facilitates the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction. Mutagenesis experiments showed that the residues that are absolutely conserved across the TRP family, including His11, Arg51, His102, and the C-terminal Tyr-Arg-Gly-Ser, may constitute the active site of mTRP. Based on these results, we propose that the transthyretin-related proteins present in diverse organisms are not functionally related to transthyretin but actually function as hydroxyisourate hydrolases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / chemistry
  • Amidohydrolases / genetics
  • Amidohydrolases / metabolism*
  • Amino Acid Sequence
  • Animals
  • Hydrolases / metabolism*
  • Mice
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Sequence Alignment
  • Urate Oxidase / metabolism*
  • Uric Acid / analogs & derivatives*
  • Uric Acid / metabolism

Substances

  • 5-hydroxyisourate
  • Uric Acid
  • Urate Oxidase
  • Hydrolases
  • Amidohydrolases
  • transthyretin-related protein, mouse