Abstract
Overproduction of the ER membrane protein Rcr1 makes Saccharomyces cerevisiae resistant to Congo red by reducing the chitin content through a unknown mechanism. By both co-immunoprecipitation and yeast two-hybrid experiments, specific interaction between Rcr1 and the ubiquitin ligase Rsp5 was found. This binding was largely mediated by a singular VPEY sequence in Rcr1 in addition to PPSY, the consensus ligand motif of the WW domains. Mutant analysis indicated that Rsp5 and other Rcr1-interacting proteins discovered in the current screen were not engaged in Congo red resistance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Endoplasmic Reticulum / metabolism*
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Endosomal Sorting Complexes Required for Transport
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Protein Binding
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Ubiquitin-Protein Ligase Complexes / genetics
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Ubiquitin-Protein Ligase Complexes / metabolism*
Substances
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Endosomal Sorting Complexes Required for Transport
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Membrane Proteins
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Rcr1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Ubiquitin-Protein Ligase Complexes
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RSP5 protein, S cerevisiae