Abstract
Neuronal gene expression is tightly regulated in developing CNS. Here, we demonstrate the anti-neural function of phosphatase SCP1 (small C-terminal domain phosphatase 1) during development. We further show that the neuron-enriched microRNA miR-124 directly targets SCP1-3' untranslated region (UTR) to suppress SCP1 expression. In developing spinal cord, expression of miR-124 and SCP1 is complementary, and miR-124 antagonism phenocopies SCP1 overexpression and vice versa. In P19 cells, miR-124 suppresses SCP1 expression and induces neurogenesis, and SCP1 counteracts this proneural activity of miR-124. Our results suggest that, during CNS development, timely down-regulation of SCP1 is critical for inducing neurogenesis, and miR-124 contributes to this process at least in part by down-regulating SCP1 expression.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / metabolism
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Animals
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Avian Proteins / antagonists & inhibitors
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Avian Proteins / genetics
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Avian Proteins / metabolism
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Base Sequence
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Cells, Cultured
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Central Nervous System / embryology*
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Central Nervous System / enzymology
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Central Nervous System / metabolism
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Chick Embryo
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Chickens
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Down-Regulation
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Humans
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Mice
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MicroRNAs / metabolism*
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Molecular Sequence Data
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Neurons / enzymology
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Neurons / metabolism*
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Phosphoprotein Phosphatases / antagonists & inhibitors*
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Phosphoprotein Phosphatases / genetics
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Phosphoprotein Phosphatases / metabolism
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Protein Phosphatase 1
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Repressor Proteins / antagonists & inhibitors*
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Transcription Factors / antagonists & inhibitors*
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Transfection
Substances
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3' Untranslated Regions
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Avian Proteins
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MicroRNAs
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RE1-silencing transcription factor
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Repressor Proteins
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Transcription Factors
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Phosphoprotein Phosphatases
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Protein Phosphatase 1