Abstract
The amino acid sequences of eleven RIPs sequenced to date have been compared in the expectation that this would be useful in the location of functionally and/or structurally important sites of these molecules. In addition to several highly conserved hydrophobic amino acids, thirteen absolutely conserved residues have been found in ricin A-chain: Tyr21, Phe24, Arg29, Tyr80, Tyr123, Gly140, Ala165, Glu177, Ala178, Arg180, Glu208, Asn209 and Trp211. The role of these residues as well as of the C-terminal region have been discussed based on the results of chemical and enzymatic modifications, site-directed mutagenesis, and deletion studies.
MeSH terms
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Abrin / genetics
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Amino Acid Sequence
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Binding Sites
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Immunotoxins*
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Lectins / genetics
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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N-Glycosyl Hydrolases*
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Plant Lectins*
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Ribosome Inactivating Proteins, Type 1
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Ribosome Inactivating Proteins, Type 2
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Ribosomes / metabolism*
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Ricin / genetics
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Saporins
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Sequence Homology, Nucleic Acid
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Trichosanthin / genetics
Substances
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Immunotoxins
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Lectins
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Plant Lectins
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Plant Proteins
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Ribosome Inactivating Proteins, Type 1
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Ribosome Inactivating Proteins, Type 2
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Ricinus communis agglutinin-1
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luffin-a protein, Luffa cylindrica
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luffin-b protein, Luffa cylindrica
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Abrin
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Trichosanthin
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Ricin
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N-Glycosyl Hydrolases
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MAP protein, Mirabilis jalapa
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Saporins
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pokeweed antiviral protein