Abstract
The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.
Publication types
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Typing Techniques
-
DNA Probes
-
Escherichia coli / classification*
-
Escherichia coli / genetics
-
Escherichia coli / metabolism
-
Escherichia coli / pathogenicity*
-
Escherichia coli Infections / microbiology
-
Escherichia coli Proteins / genetics
-
Escherichia coli Proteins / metabolism
-
Humans
-
Oligonucleotide Array Sequence Analysis / methods*
-
Polymerase Chain Reaction
-
Sensitivity and Specificity
-
Virulence
Substances
-
DNA Probes
-
Escherichia coli Proteins