Abstract
ACC-4, an omega loop mutant (Val(211)-->Gly) of the Hafnia alvei-derived cephalosporinase ACC-1, was encoded by an Escherichia coli plasmid. The genetic environment of bla(ACC-4) shared similarities with plasmidic regions carrying bla(ACC-1). Kinetics of beta-lactam hydrolysis and levels of resistance to beta-lactams showed that ACC-4 was more effective than ACC-1 against expanded-spectrum cephalosporins.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Cephalosporinase / genetics*
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Cephalosporinase / isolation & purification
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Culture Media
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DNA Primers
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Drug Resistance, Bacterial / genetics
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Escherichia coli / enzymology*
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Escherichia coli / genetics*
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Kinetics
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Models, Molecular
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Molecular Sequence Data
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Plasmids / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Spectrophotometry, Ultraviolet
Substances
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Anti-Bacterial Agents
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Culture Media
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DNA Primers
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Cephalosporinase