Abstract
Selective inactivation of cytosolic thiolase by 2-alkynoyl-CoA via its intrinsic isomerase activity was studied, which provides an example for rationally developing mechanism-based inhibitors based on a side activity of the enzyme, and may become a supplemental method for better treatment of cardiovascular disease and cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyl Coenzyme A / chemistry*
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Acetyl Coenzyme A / metabolism*
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Amino Acids / chemistry
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Amino Acids / metabolism
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Animals
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Catalysis
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Cell Line
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Enzyme Activation
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Isomerism
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Kinetics
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Liver / enzymology
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Molecular Structure
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism
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Rats
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Substrate Specificity
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Sulfhydryl Compounds / metabolism*
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Tandem Mass Spectrometry
Substances
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Amino Acids
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Peptide Fragments
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Sulfhydryl Compounds
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Acetyl Coenzyme A