Abstract
We describe here the cloning and characterization of a novel mouse homeodomain-interacting protein kinase (HIPK)-like gene, Hipk4. Hipk4 is expressed in lung and in white adipose tissue and encodes a 616 amino acid protein that includes a serine/threonine kinase domain. We demonstrate that HIPK4 could phosphorylate human p53 protein at serine 9, both in vitro and in vivo. Among known p53-responsive promoters, activity of the human survivin promoter, which is repressed by p53, was decreased by HIPK4 in p53 functional A549 cells. Human BCL2-associated X protein-promoter activity was not affected. These findings suggest that phosphorylation of p53 at serine 9 is important for p53 mediated transcriptional repression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Catalysis
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Cell Differentiation
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Cell Proliferation
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Cloning, Molecular
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DNA, Complementary / metabolism
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Humans
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Inhibitor of Apoptosis Proteins
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Mice
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism
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Molecular Sequence Data
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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Promoter Regions, Genetic
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Protein Serine-Threonine Kinases / analysis
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Sequence Alignment
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Serine / genetics
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Serine / metabolism*
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Survivin
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Tumor Suppressor Protein p53 / metabolism*
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bcl-2-Associated X Protein / genetics
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bcl-2-Associated X Protein / metabolism
Substances
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BAX protein, human
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BIRC5 protein, human
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DNA, Complementary
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Inhibitor of Apoptosis Proteins
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Microtubule-Associated Proteins
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Neoplasm Proteins
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Survivin
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein
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Serine
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HIPK4 protein, human
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Protein Serine-Threonine Kinases