Abstract
Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y RNAs. Unexpectedly, Y5 RNA uniquely copurified ribosomal protein L5 and its binding partner 5S RNA. These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5-5S RNPs establish 5S RNA as a target of quality control.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Affinity Labels
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Autoantigens / genetics*
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Autoantigens / isolation & purification
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Autoantigens / metabolism*
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Carrier Proteins / metabolism
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Cell Line
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DNA-Binding Proteins
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Genetic Variation
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Humans
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Neoplasm Proteins / metabolism
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RNA Splicing Factors
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RNA, Ribosomal, 5S / genetics*
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RNA, Ribosomal, 5S / metabolism*
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RNA, Small Cytoplasmic / genetics*
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RNA, Small Cytoplasmic / isolation & purification
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RNA, Small Cytoplasmic / metabolism*
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RNA, Untranslated / genetics*
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RNA, Untranslated / metabolism*
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RNA-Binding Proteins
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins
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Ribonucleoproteins / genetics*
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Ribonucleoproteins / isolation & purification
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Ribonucleoproteins / metabolism*
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Ribosomal Proteins / genetics
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Ribosomal Proteins / isolation & purification
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Ribosomal Proteins / metabolism
Substances
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Affinity Labels
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Autoantigens
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Carrier Proteins
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DNA-Binding Proteins
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IFIT5 protein, human
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Neoplasm Proteins
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RNA Splicing Factors
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RNA, Ribosomal, 5S
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RNA, Small Cytoplasmic
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RNA, Untranslated
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RNA-Binding Proteins
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RO60 protein, human
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Recombinant Fusion Proteins
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Repressor Proteins
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Ribonucleoproteins
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Ribosomal Proteins
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poly-U binding splicing factor 60KDa
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ribosomal protein L5