Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control

Genes Dev. 2007 Dec 1;21(23):3067-72. doi: 10.1101/gad.1603907.

Abstract

Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y RNAs. Unexpectedly, Y5 RNA uniquely copurified ribosomal protein L5 and its binding partner 5S RNA. These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5-5S RNPs establish 5S RNA as a target of quality control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Affinity Labels
  • Autoantigens / genetics*
  • Autoantigens / isolation & purification
  • Autoantigens / metabolism*
  • Carrier Proteins / metabolism
  • Cell Line
  • DNA-Binding Proteins
  • Genetic Variation
  • Humans
  • Neoplasm Proteins / metabolism
  • RNA Splicing Factors
  • RNA, Ribosomal, 5S / genetics*
  • RNA, Ribosomal, 5S / metabolism*
  • RNA, Small Cytoplasmic / genetics*
  • RNA, Small Cytoplasmic / isolation & purification
  • RNA, Small Cytoplasmic / metabolism*
  • RNA, Untranslated / genetics*
  • RNA, Untranslated / metabolism*
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins / isolation & purification
  • Ribonucleoproteins / metabolism*
  • Ribosomal Proteins / genetics
  • Ribosomal Proteins / isolation & purification
  • Ribosomal Proteins / metabolism

Substances

  • Affinity Labels
  • Autoantigens
  • Carrier Proteins
  • DNA-Binding Proteins
  • IFIT5 protein, human
  • Neoplasm Proteins
  • RNA Splicing Factors
  • RNA, Ribosomal, 5S
  • RNA, Small Cytoplasmic
  • RNA, Untranslated
  • RNA-Binding Proteins
  • RO60 protein, human
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Ribonucleoproteins
  • Ribosomal Proteins
  • poly-U binding splicing factor 60KDa
  • ribosomal protein L5