Abstract
A full-length cDNA encoding ribosome-inactivating/antiviral protein (RIP/AVP)from the leaves of Bougainvillea x buttiana was isolated. The cDNA consisted of 1364 nucleotides with an open reading frame (ORF)of 960 nucleotides encoding a 35.49 kDa protein of 319 amino acids. The deduced amino acid sequence has a putative active domain conserved in RIPs/AVPs and shows a varying phylogenetic relationship to the RIPs from other plant species. The deduced protein has been designated BBAP1 (Bougainvillea x buttiana antiviral protein1). The ORF was cloned into an expression vector and expressed in E.coli as a fusion protein of approximately 78 kDa. The cleaved and purified recombinant BBAP1 exhibited ribosome-inhibiting rRNA N-glycosidase activity,and imparted a high level of resistance against the tobacco mosaic virus (TMV).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antiviral Agents / chemistry
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Antiviral Agents / isolation & purification
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Base Sequence
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Cloning, Molecular
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DNA, Complementary / genetics
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Escherichia coli / genetics
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Gene Expression*
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Genes, Plant
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Glycoside Hydrolases / analysis
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Glycoside Hydrolases / genetics
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Glycoside Hydrolases / metabolism
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Molecular Sequence Data
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Nyctaginaceae / anatomy & histology
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Nyctaginaceae / chemistry
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Nyctaginaceae / genetics*
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Open Reading Frames
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Phylogeny
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Plant Leaves / chemistry
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Plant Leaves / genetics
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Plant Leaves / growth & development
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Plant Leaves / metabolism
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / isolation & purification
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Plant Proteins / metabolism
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Protein Sorting Signals
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Protein Structure, Tertiary
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Protein Synthesis Inhibitors / chemistry
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Protein Synthesis Inhibitors / isolation & purification
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Protein Synthesis Inhibitors / metabolism
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Recombinant Fusion Proteins / analysis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Ribosome Inactivating Proteins / chemistry
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Ribosome Inactivating Proteins / genetics*
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Ribosome Inactivating Proteins / isolation & purification
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Ribosome Inactivating Proteins / metabolism
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Tobacco Mosaic Virus / physiology
Substances
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Antiviral Agents
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DNA, Complementary
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Plant Proteins
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Protein Sorting Signals
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Protein Synthesis Inhibitors
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Recombinant Fusion Proteins
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Glycoside Hydrolases
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Ribosome Inactivating Proteins