Current Good Manufacturing Practices (cGMP) for botanicals stipulates the use of appropriate methods for identification of raw materials. Due to natural variability, chemical analysis of plant material is a great challenge and requires special approaches. This paper presents a comprehensive proposal to the process of validating qualitative high-performance thin-layer chromatographic (HPTLC) methods, proving that such methods are suitable for the purpose. The steps of the validation process are discussed and illustrated with examples taken from a project aiming at validation of methods for identification of green tea leaf, ginseng root, eleuthero root, echinacea root, black cohosh rhizome, licorice root, kava root, milk thistle aerial parts, feverfew aerial parts, and ginger root. The appendix of the paper, which includes complete documentation and method write-up for those plants, is available on the J. AOAC Int. Website (http://www.atypon-link.com/AOAC/loi/jaoi).