Flow cytometry of bacterial membrane potential and permeability

Howard M Shapiro

doi:10.1007/978-1-59745-246-5_14

Flow cytometry of bacterial membrane potential and permeability

Methods Mol Med. 2008:142:175-86. doi: 10.1007/978-1-59745-246-5_14.

Author

Howard M Shapiro¹

Affiliation

¹ The Center for Microbial Cytometry and Howard M Shapiro, West Newton, MA, USA.

PMID: 18437314
DOI: 10.1007/978-1-59745-246-5_14

Abstract

This chapter describes reliable flow cytometric methods for assessment of two important physiologic characteristics of bacteria, membrane potential and membrane permeability, which can provide indications of the effects of antimicrobial agents on microorganisms.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Physiological Phenomena / drug effects
Carbocyanines / metabolism
Cell Membrane Permeability / drug effects*
Flow Cytometry / methods*
Fluorescent Dyes / metabolism
Membrane Potentials / drug effects*
Mycobacterium smegmatis / drug effects
Mycobacterium smegmatis / physiology
Staphylococcus aureus / drug effects
Staphylococcus aureus / physiology

Substances

Anti-Bacterial Agents
Carbocyanines
Fluorescent Dyes
TO-PRO-3
3,3'-diethyloxacarbocyanine