Abstract
A proteolysed form of fructose 1,6-bisphosphatase (Fru-P2ase) has been detected and characterized in human term placenta. The extract was found to contain very low levels of activity with an alkaline pH optimum. Western blotting demonstrated a polypeptide of Mr 26,000, instead of the subunit of Mr 36,000 observed in native mammalian Fru-P2ases.
MeSH terms
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Blotting, Western
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Electrophoresis, Polyacrylamide Gel
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Female
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Fructose-Bisphosphatase / isolation & purification
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Fructose-Bisphosphatase / metabolism*
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Humans
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Hydrogen-Ion Concentration
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Kinetics
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Macromolecular Substances
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Molecular Weight
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Placenta / enzymology*
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Pregnancy
Substances
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Macromolecular Substances
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Fructose-Bisphosphatase