129/SvJ mice have mutated CD23 and hyper IgE

Cell Immunol. 2009;254(2):124-34. doi: 10.1016/j.cellimm.2008.08.003. Epub 2008 Oct 1.

Abstract

CD23, the low affinity IgE receptor, is hypothesized to function as a negative regulator of IgE production. Upon discovering reduced CD23 surface levels in 129/SvJ inbred mice, we sought to further investigate 129/SvJ CD23 and to examine its influence on IgE levels. Five amino acid substitutions were found in 129/SvJ CD23. Identical mutations were also observed in CD23 from New Zealand Black and 129P1/ReJ mice. 129/SvJ B cells proliferated more rapidly than those from BALB/c after stimulation with IL-4 and CD40 ligand trimer. However, in vitro IgE levels in supernatants from stimulated 129/SvJ B cells were significantly reduced. Contrary to the in vitro findings, the 129/SvJ CD23 mutations correlated with a hyper IgE phenotype in vivo and 129/SvJ were able to clear Nippostrongylus brasiliensis infection more rapidly than either BALB/c or C57BL/6. Overall, this study further suggests that CD23 is an important regulatory factor for IgE production.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Formation / immunology
  • Binding Sites
  • Cell Membrane / immunology
  • Cells, Cultured
  • Conserved Sequence
  • Gene Expression Regulation
  • Immunoglobulin E / immunology*
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Mutation / genetics*
  • RNA, Messenger / genetics
  • Receptors, IgE / blood
  • Receptors, IgE / chemistry
  • Receptors, IgE / genetics*
  • Receptors, IgE / immunology*
  • Sequence Alignment

Substances

  • RNA, Messenger
  • Receptors, IgE
  • Immunoglobulin E