RNA-Seq: a revolutionary tool for transcriptomics

Nat Rev Genet. 2009 Jan;10(1):57-63. doi: 10.1038/nrg2484.

Abstract

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

Publication types

  • Review

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Exons
  • Gene Expression Profiling / methods*
  • Humans
  • Models, Genetic
  • Molecular Sequence Data
  • RNA / analysis*
  • Sequence Analysis, RNA / methods*
  • Transcription, Genetic*

Substances

  • RNA