Abstract
Escherichia coli (E. coli) that expresses galactose-reactive lectins, like K88 adhesin, causes high mortality among piglets. Carbohydrates that compete for adhesion could serve as an alternative for disease prevention. Porcine serum albumin (PSA) was modified by non-enzymatic glycation with lactose to produce PSA-Lac or PSA-Glc beta (1-4) Gal, as confirmed by reduction of available free amino groups, increased molecular mass and by Ricinus communis lectin recognition. E. coli K88 binds to PSA-Lac treatments containing three and four lactoses, respectively. In addition, PSA-Lac partially inhibited K88 strain adherence to mucins. These results suggest that neoglycoconjugates obtained by non-enzymatic glycation of proteins may serve in the prophylaxis of piglets' diarrhea.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Escherichia coli / chemistry
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Adhesins, Escherichia coli / metabolism*
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Amines
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Animals
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / metabolism*
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Bacterial Adhesion / drug effects
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Electrophoresis
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Escherichia coli / cytology
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Escherichia coli / drug effects
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Escherichia coli / metabolism
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Escherichia coli Proteins / chemistry
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Escherichia coli Proteins / metabolism*
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Fimbriae Proteins / chemistry
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Fimbriae Proteins / metabolism*
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Glycosylation / drug effects
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Lactose / metabolism
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Molecular Weight
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Plant Lectins / pharmacology
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Serum Albumin / chemistry
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Serum Albumin / isolation & purification
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Serum Albumin / metabolism*
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Serum Albumin / pharmacology
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Spectrometry, Fluorescence
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Sus scrofa
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Tryptophan / metabolism
Substances
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Adhesins, Escherichia coli
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Amines
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Antigens, Bacterial
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Escherichia coli Proteins
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K88 antigen, E coli
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Plant Lectins
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Ricinus communis agglutinin-1
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Serum Albumin
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Fimbriae Proteins
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Tryptophan
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Lactose