ICSBP-mediated immune protection against BCR-ABL-induced leukemia requires the CCL6 and CCL9 chemokines

Blood. 2009 Apr 16;113(16):3813-20. doi: 10.1182/blood-2008-07-167189. Epub 2009 Jan 26.

Abstract

Interferon (IFN) is effective at inducing complete remissions in patients with chronic myelogenous leukemia (CML), and evidence supports an immune mechanism. Here we show that the type I IFNs (alpha and beta) regulate expression of the IFN consensus sequence-binding protein (ICSBP) in BCR-ABL-transformed cells and as shown previously for ICSBP, induce a vaccine-like immunoprotective effect in a murine model of BCR-ABL-induced leukemia. We identify the chemokines CCL6 and CCL9 as genes prominently induced by the type I IFNs and ICSBP, and demonstrate that these immunomodulators are required for the immunoprotective effect of ICSBP expression. Insights into the role of these chemokines in the antileukemic response of IFNs suggest new strategies for immunotherapy of CML.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemokines, CC / biosynthesis
  • Chemokines, CC / immunology*
  • Disease Models, Animal
  • Female
  • Gene Expression Regulation, Leukemic / drug effects*
  • Gene Expression Regulation, Leukemic / immunology
  • Genes, abl / immunology*
  • Humans
  • Interferon Regulatory Factors / biosynthesis
  • Interferon Regulatory Factors / genetics
  • Interferon Regulatory Factors / immunology*
  • Interferon Type I / immunology
  • Interferon Type I / metabolism
  • Interferon Type I / pharmacology*
  • K562 Cells
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / immunology*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
  • Macrophage Inflammatory Proteins / biosynthesis
  • Macrophage Inflammatory Proteins / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout

Substances

  • Ccl9 protein, mouse
  • Chemokines, CC
  • Interferon Regulatory Factors
  • Interferon Type I
  • Macrophage Inflammatory Proteins
  • interferon regulatory factor-8
  • Ccl6 protein, mouse