Relationship between nuclear sequestration of PLCzeta and termination of PLCzeta-induced Ca2+ oscillations in mouse eggs

Cell Calcium. 2008 Oct;44(4):400-10. doi: 10.1016/j.ceca.2008.02.003.

Abstract

Phospholipase C-zeta (PLCzeta), a strong candidate of the egg-activating sperm factor, causes long-lasting series of Ca2+ spikes and thereby egg activation when expressed in mouse eggs by injection of cRNA. PLCzeta moves into the formed pronucleus (PN), and Ca2+ spikes disappear at PN stage. Relationship between nuclear transLocation of PLCzeta and cessation of Ca2+ oscillations was addressed using various concentrations of wild-type RNA and point mutant K377E RNA having the comparable expression efficiency. PLCzeta-induced Ca2+ spikes with 20-30 min intervals similar to those at fertilization ceased between 50 min before and 15 min after the time of complete PN formation (TPN) approximately 5 h after the first Ca2+ spike, whereas Ca2+ oscillations induced by K377E lacking nuclear translocation ability continued over 9 h. Formation of the nuclear envelope (NE) began 50-60 min before T(PN), visualized by labeling the endoplasmic reticulum network with fluorescent dye Dil and ER-targeting protein ER-DsRed2. PLCzeta entered the PN as soon as the NE was formed, and accumulated in enlarging PN. After in vitro fertilization as normal as possible, the last Ca2+ spike occurred between 25 min before and 35 min after initiation of NE formation in most cases. Thus, sequestration of PLCzeta into the PN participates in termination of Ca2+ oscillations at the interphase in the mouse 1-cell embryo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Cell Nucleus / enzymology
  • Cell Nucleus / metabolism*
  • Female
  • Fertilization / physiology
  • Male
  • Mice
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism*
  • Oocytes / cytology
  • Oocytes / enzymology
  • Oocytes / metabolism*
  • Phosphoinositide Phospholipase C / genetics
  • Phosphoinositide Phospholipase C / metabolism*
  • Spermatozoa / enzymology*

Substances

  • Mutant Proteins
  • Phosphoinositide Phospholipase C
  • Plcz1 protein, mouse
  • Calcium