A novel cardiotropic murine adenovirus representing a distinct species of mastadenoviruses

J Virol. 2009 Jun;83(11):5749-59. doi: 10.1128/JVI.02281-08. Epub 2009 Mar 18.

Abstract

During cell culture isolation experiments to recover Dobrava hantavirus from a suspension of liver from a striped field mouse (Apodemus agrarius), an unknown virus was coisolated. Atypically for hantaviruses, it had extensive cytopathic effects. Using a random PCR approach, it was identified as a novel murine adenovirus, MAdV-3 (for MAdV type 3). A plaque-purified virus clone was prepared and further characterized. The complete genome sequence of MAdV-3 was determined to be 30,570 bp in length. Sequence comparisons to other adenovirus species revealed highest similarity to MAdV-1, the representative of the murine adenovirus A species. However, substantial differences were found in the E1, E3, and E4 genomic regions. The phylogenetic distance of MAdV-3 amino acid sequences for pVIII, protease, polymerase, and hexon from MAdV-1 is markedly higher than 0.1 exchange per position, and, based on our cross-neutralization experiments, MAdV-3 and MAdV-1 can be regarded as different serotypes. Therefore, we propose to classify MAdV-3 as the first isolate of a novel adenovirus species, designated murine adenovirus C (MAdV-C). The novel MAdV-3 virus is not only genetically and serologically distinct from MAdV-1 but also shows a unique organ tropism in infected mice. In contrast to MAdV-1, the virus was not detectable in brain but predominantly infected heart tissue. Thus, infection of mice with cardiotropic MAdV-3 might be an interesting animal model of adenovirus-induced myocarditis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Adenoviridae / isolation & purification
  • Adenoviridae / metabolism*
  • Alternative Splicing / genetics
  • Animals
  • Base Sequence
  • Chlorocebus aethiops
  • DNA, Viral / genetics
  • Female
  • Genome, Viral / genetics
  • Mastadenovirus / genetics
  • Mastadenovirus / isolation & purification
  • Mastadenovirus / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Phylogeny
  • Sequence Alignment
  • Vero Cells

Substances

  • DNA, Viral