Abstract
A weblogo has been generated from the sequences surrounding 433 Ser/Thr protein residues whose phosphorylation by protein kinase CK2 had been previously validated ("bona fide" CK2 phosphosites). This has been compared to the weblogo extracted from 2275 putative CK2 phosphosites displaying the motif pS/pT-x1-x2-D/E/pS (where x1 not=P) present in the human phosphoElm database including 10899 naturally occurring phosphosites. The two weblogos are strikingly similar supporting the notion that indeed the 2275 putative sites (accounting for 20.9% of the whole phosphoproteome they belong to), or at least the great majority of these are generated by CK2. This conclusion has been corroborated by the random validation of 8 of such putative CK2 sites (belonging to 5 different proteins) as real targets of CK2 in vitro and/or in cells, leading to the inclusion into the repertoire of bona fide CK2 targets of 5 new entries, namely: oxidative stress-responsive kinase-1, anthrax toxin receptor 1, hepatoma derived growth factor, EpsinR and BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Vesicular Transport / genetics
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Adaptor Proteins, Vesicular Transport / metabolism
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Amino Acid Sequence
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Animals
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Casein Kinase II / chemistry
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Casein Kinase II / genetics
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Casein Kinase II / metabolism*
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Cell Line
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Humans
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Isoenzymes / chemistry
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Isoenzymes / genetics
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Isoenzymes / metabolism*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Phosphoproteins / analysis*
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism
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Proteome / analysis*
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Proteomics / methods*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / metabolism
Substances
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Adaptor Proteins, Vesicular Transport
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BNIP3L protein, human
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CLINT1 protein, human
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Isoenzymes
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Membrane Proteins
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Phosphoproteins
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Proteome
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Proto-Oncogene Proteins
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Receptors, Cell Surface
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Recombinant Fusion Proteins
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Tumor Suppressor Proteins
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Casein Kinase II
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Protein Serine-Threonine Kinases