De novo design of a beta alpha beta motif

Huanhuan Liang; Hao Chen; Keqiang Fan; Ping Wei; Xianrong Guo; Changwen Jin; Chen Zeng; Chao Tang; Luhua Lai

doi:10.1002/anie.200805476

De novo design of a beta alpha beta motif

Angew Chem Int Ed Engl. 2009;48(18):3301-3. doi: 10.1002/anie.200805476.

Authors

Huanhuan Liang¹, Hao Chen, Keqiang Fan, Ping Wei, Xianrong Guo, Changwen Jin, Chen Zeng, Chao Tang, Luhua Lai

Affiliation

¹ College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

PMID: 19347908
DOI: 10.1002/anie.200805476

Abstract

A designer monomeric protein with a beta alpha beta fold--two parallel beta strands connected by an alpha helix (see structure)--was constructed solely from coded amino acids. The high thermal stability of the structure is due to a large extent to tryptophan-tryptophan interactions between the two beta strands.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Motifs* / genetics
Amino Acid Sequence
Molecular Sequence Data
Mutation
Protein Folding*
Proteins / chemistry*
Proteins / genetics

Substances

Proteins