Sequencing and computational analysis of complete genome sequences of Citrus yellow mosaic badna virus from acid lime and pummelo

Virus Genes. 2009 Aug;39(1):137-40. doi: 10.1007/s11262-009-0367-9. Epub 2009 May 15.

Abstract

Citrus yellow mosaic badna virus (CMBV), a member of the Family Caulimoviridae, Genus Badnavirus, is the causative agent of Citrus mosaic disease in India. Although the virus has been detected in several citrus species, only two full-length genomes, one each from Sweet orange and Rangpur lime, are available in publicly accessible databases. In order to obtain a better understanding of the genetic variability of the virus in other citrus mosaic-affected citrus species, we performed the cloning and sequence analysis of complete genomes of CMBV from two additional citrus species, Acid lime and Pummelo. We show that CMBV genomes from the two hosts share high homology with previously reported CMBV sequences and hence conclude that the new isolates represent variants of the virus present in these species. Based on in silico sequence analysis, we predict the possible function of the protein encoded by one of the five ORFs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Badnavirus / classification
  • Badnavirus / genetics*
  • Badnavirus / isolation & purification*
  • Citrus / virology*
  • Citrus aurantiifolia / virology*
  • DNA, Viral / chemistry
  • DNA, Viral / genetics*
  • Genome, Viral*
  • India
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Analysis, DNA*
  • Sequence Homology
  • Viral Proteins / genetics

Substances

  • DNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/EU489744
  • GENBANK/EU489745