Abstract
Objectives:
The study aim was to establish conditions for stabilization the activity of fructose-1,6-bisphosphatase (FBP-1) in stored urine.
Design and methods:
The FBP-1 was determined by the method Kepka et. al in a collected fraction of purified urine.
Results:
EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine.
Conclusion:
At optimal conditions urine may be stored up to 7 days at a temperature of 4 degrees C.
MeSH terms
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Chelating Agents / pharmacology
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Child
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Creatinine / urine
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Edetic Acid / pharmacology*
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Enzyme Stability / drug effects
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Female
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Fructose-Bisphosphatase / metabolism*
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Fructose-Bisphosphatase / urine*
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Humans
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Hydrogen-Ion Concentration
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Male
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Mercaptoethanol / pharmacology*
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Preservation, Biological / methods
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Temperature
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Time Factors
Substances
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Chelating Agents
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Mercaptoethanol
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Edetic Acid
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Creatinine
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Fructose-Bisphosphatase