Abstract
Chemokines are important in HSE development in the CNS but underlying regulatory events are unknown. Two-hybrid binding assays identified that intercellular adhesion molecule 5 (ICAM-5), an immune modulator in the CNS, interacted with neurovirulence factor, UOL, of HSV-1. Viral load and interleukin levels were similar in UOL deletion virus (DeltaUOL), and wild type virus infected mouse brains. However, higher numbers of lymphocytes, but unaltered soluble ICAM-5 and chemokine levels were detected in DeltaUOL infected mouse brains. In contrast, lower lymphocyte numbers, reduced soluble ICAM-5, and higher chemokine levels were detected in wild type virus infected brains. Our results suggest that ICAM-5 plays a critical role in modulating chemokine production in the CNS.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Brain / immunology
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Brain / metabolism*
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Brain / virology
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Cells, Cultured
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Chemokines / metabolism*
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Cytokines / metabolism
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Disease Models, Animal
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Down-Regulation / immunology
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Encephalitis, Herpes Simplex / immunology
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Encephalitis, Herpes Simplex / metabolism*
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Encephalitis, Herpes Simplex / physiopathology
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Female
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Herpesvirus 1, Human / immunology
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Herpesvirus 1, Human / metabolism
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Immunologic Factors / metabolism
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Interleukins / metabolism
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Lymphocyte Activation / immunology
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Lymphocyte Count
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Lymphocytes / immunology
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Lymphocytes / metabolism*
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Membrane Glycoproteins / metabolism*
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Mice
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Nerve Tissue Proteins / metabolism*
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Up-Regulation / immunology
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Viral Load
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Virulence Factors / metabolism
Substances
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Chemokines
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Cytokines
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Icam5 protein, mouse
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Immunologic Factors
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Interleukins
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Membrane Glycoproteins
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Nerve Tissue Proteins
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Virulence Factors