Abstract
Translational control plays an important role in cell growth and tumorigenesis. Cap-dependent translation initiation of mammalian mRNAs with structured 5'UTRs requires the DExH-box protein, DHX29, in vitro. Here we show that DHX29 is important for translation in vivo. Down-regulation of DHX29 leads to impaired translation, resulting in disassembly of polysomes and accumulation of mRNA-free 80S monomers. DHX29 depletion also impedes cancer cell growth in culture and in xenografts. Thus, DHX29 is a bona fide translation initiation factor that potentially can be exploited as a target to inhibit cancer cell growth.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Untranslated Regions
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Animals
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Cell Proliferation*
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Down-Regulation
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HeLa Cells
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Humans
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Mice
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Mice, Nude
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Neoplasm Transplantation
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Neoplasms / enzymology
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Neoplasms / etiology*
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Neoplasms / genetics
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Neoplasms / pathology
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Peptide Chain Initiation, Translational / physiology*
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RNA Helicases / antagonists & inhibitors
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RNA Helicases / genetics
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RNA Helicases / metabolism*
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RNA Interference
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Small Interfering / genetics
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Ribosome Subunits, Small, Eukaryotic / metabolism
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Transplantation, Heterologous
Substances
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5' Untranslated Regions
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RNA, Messenger
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RNA, Small Interfering
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DHX29 protein, human
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RNA Helicases