Ngaingan virus, a macropod-associated rhabdovirus, contains a second glycoprotein gene and seven novel open reading frames

Aneta Gubala; Steven Davis; Richard Weir; Lorna Melville; Chris Cowled; Peter Walker; David Boyle

doi:10.1016/j.virol.2009.12.013

Ngaingan virus, a macropod-associated rhabdovirus, contains a second glycoprotein gene and seven novel open reading frames

Virology. 2010 Mar 30;399(1):98-108. doi: 10.1016/j.virol.2009.12.013. Epub 2010 Jan 20.

Authors

Aneta Gubala¹, Steven Davis², Richard Weir³, Lorna Melville³, Chris Cowled⁴, Peter Walker⁵, David Boyle⁴

Affiliations

¹ CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia; School of Chemistry and Molecular Sciences, University of Queensland, St. Lucia, Queensland, Australia; Human Protection and Performance Division, Defence Science and Technology Organisation, Melbourne, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia. Electronic address: ania.gubala@dsto.defence.gov.au.
² Northern Territory Department of Regional Development, Primary Industry, Fisheries and Resources, Berrimah Veterinary Laboratories, Berrimah, Northern Territory, Australia.
³ Northern Territory Department of Regional Development, Primary Industry, Fisheries and Resources, Berrimah Veterinary Laboratories, Berrimah, Northern Territory, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia.
⁴ CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia.
⁵ CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia.

PMID: 20089287
DOI: 10.1016/j.virol.2009.12.013

Abstract

Ngaingan virus (NGAV) was isolated from a pool of biting midges that were collected in the tropics of northern Australia. Reported here is the full-length sequence of the NGAV genome, which, at over 15.7 kb, is the largest in any rhabdovirus described to date and contains 13 genes, the highest number of genes observed in any (-) ssRNA virus. Seven of these putative genes show no significant homology to known proteins. Like viruses in the genus Ephemerovirus, NGAV possesses a second glycoprotein gene (G(NS)). Phylogenetic analyses, however, place NGAV within the yet to be classified "Hart Park" group containing Wongabel and Flanders viruses, which do not contain a second glycoprotein gene. Screening of various animal sera from northern Australia has indicated that NGAV is currently circulating in macropods (wallabies, wallaroos and kangaroos), highlighting the need for further studies to determine its potential to cause disease in these species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Ceratopogonidae / virology
Chromosome Mapping
Genes, Viral / genetics*
Genome, Viral / genetics
Glycoproteins / genetics
Molecular Sequence Data
Neutralization Tests
Nucleocapsid Proteins / genetics
Open Reading Frames / genetics*
Phylogeny
Rhabdoviridae / genetics*
Sequence Analysis, Protein
Viral Envelope Proteins / genetics*
Viral Matrix Proteins / genetics
Viral Proteins / genetics
Virus Replication / genetics

Substances

Glycoproteins
Nucleocapsid Proteins
Viral Envelope Proteins
Viral Matrix Proteins
Viral Proteins

Associated data

GENBANK/FJ715959