Protein kinase C-related kinase targets nuclear localization signals in a subset of class IIa histone deacetylases

FEBS Lett. 2010 Mar 19;584(6):1103-10. doi: 10.1016/j.febslet.2010.02.057. Epub 2010 Feb 24.

Abstract

Class IIa histone deacetylases (HDACs) -4, -5, -7 and -9 undergo signal-dependent nuclear export upon phosphorylation of conserved serine residues that are targets for 14-3-3 binding. Little is known of other mechanisms for regulating the subcellular distribution of class IIa HDACs. Using a biochemical purification strategy, we identified protein kinase C-related kinase-2 (PRK2) as an HDAC5-interacting protein. PRK2 and the related kinase, PRK1, phosphorylate HDAC5 at a threonine residue (Thr-292) positioned within the nuclear localization signal (NLS) of the protein. HDAC7 and HDAC9 contain analogous sites that are phosphorylated by PRK, while HDAC4 harbors a non-phosphorylatable alanine residue at this position. We provide evidence to suggest that the unique phospho-acceptor cooperates with the 14-3-3 target sites to impair HDAC nuclear import.

MeSH terms

  • 14-3-3 Proteins / metabolism
  • Active Transport, Cell Nucleus / physiology
  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Catalytic Domain
  • Cells, Cultured
  • Chlorocebus aethiops
  • Consensus Sequence
  • Histone Deacetylases / chemistry*
  • Histone Deacetylases / metabolism*
  • Humans
  • Models, Biological
  • Nuclear Localization Signals / metabolism*
  • Phosphorylation
  • Protein Binding
  • Protein Interaction Mapping
  • Protein Kinase C / metabolism*

Substances

  • 14-3-3 Proteins
  • Nuclear Localization Signals
  • protein kinase N
  • Protein Kinase C
  • Histone Deacetylases