Abstract
By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acids / chemistry
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Amino Acids / metabolism*
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Base Sequence
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Codon, Terminator
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DNA, Bacterial / genetics
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Escherichia coli / genetics*
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Escherichia coli / metabolism*
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Escherichia coli Proteins / chemistry
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Escherichia coli Proteins / genetics*
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Escherichia coli Proteins / metabolism*
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Genes, Bacterial
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Lysine / analogs & derivatives
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Lysine / chemistry
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Lysine / metabolism
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Models, Molecular
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Molecular Sequence Data
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Protein Processing, Post-Translational
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Ribosomal Proteins / chemistry
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism
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Spectrometry, Mass, Electrospray Ionization
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Systems Biology
Substances
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Amino Acids
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Codon, Terminator
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DNA, Bacterial
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Escherichia coli Proteins
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Recombinant Fusion Proteins
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Ribosomal Proteins
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ribosomal protein L11
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Green Fluorescent Proteins
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N-epsilon-acetyllysine
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Lysine