The aim of this study was to investigate whether Escherichia coli O157 is present in/on raw beef in Serbia. Correlated faecal and carcasses samples from 115 slaughtered cattle plus 26 uncorrelated carcass samples were examined. E. coli O157 detection and identification was performed using selective enrichment and immunomagnetic separation followed by selective media-plating and biochemical tests. The E. coli O157 occurrences were 2.6% in faeces and 2.8% on carcasses. The E. coli O157 occurrences were 0%, 6.2% and 2.1%, respectively, in 106 samples of beef trimmings, 48 samples of minced beef and 48 samples of batter intended for production of raw, fermented sausages. The results confirmed that faecal contamination is very important for the occurrence of E. coli O157 on beef carcasses. Furthermore, the present study revealed occasional presence of the pathogen in raw materials used for producing raw, fermented beef sausages.