Complete nucleotide sequence and genome organization of Olive latent virus 3, a new putative member of the family Tymoviridae

Virus Res. 2010 Sep;152(1-2):10-8. doi: 10.1016/j.virusres.2010.05.010. Epub 2010 May 31.

Abstract

The complete nucleotide sequence and the genome organization were determined of a putative new member of the family Tymoviridae, tentatively named Olive latent virus 3 (OLV-3), recovered in southern Italy from a symptomless olive tree. The sequenced ssRNA genome comprises 7148 nucleotides excluding the poly(A) tail and contains four open reading frames (ORFs). ORF1 encodes a polyprotein of 221.6kDa in size, containing the conserved signatures of the methyltransferase (MTR), papain-like protease (PRO), helicase (HEL) and RNA-dependent RNA polymerase (RdRp) domains of the replication-associated proteins of positive-strand RNA viruses. ORF2 overlaps completely ORF1 and encodes a putative protein of 43.33kDa showing limited sequence similarity with the putative movement protein of Maize rayado fino virus (MRFV). ORF3 codes for a protein with predicted molecular mass of 28.46kDa, identified as the coat protein (CP), whereas ORF4 overlaps ORF3 and encodes a putative protein of 16kDa with sequence similarity to the p16 and p31 proteins of Citrus sudden death-associated virus (CSDaV) and Grapevine fleck virus (GFkV), respectively. Within the family Tymoviridae, OLV-3 genome has the closest identity level (49-52%) with members of the genus Marafivirus, from which, however, it differs because of the diverse genome organization and the presence of a single type of CP subunits.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Genome, Viral*
  • Molecular Sequence Data
  • Olea / virology
  • Open Reading Frames
  • Phylogeny
  • Sequence Alignment
  • Tymoviridae / chemistry
  • Tymoviridae / classification*
  • Tymoviridae / genetics*
  • Tymoviridae / isolation & purification
  • Viral Proteins / chemistry
  • Viral Proteins / genetics

Substances

  • Viral Proteins