Abstract
Bats are increasingly being recognized as important natural reservoirs of different viruses. Adeno-associated viruses (AAVs) are widely distributed in primates and their distribution in bats is unknown. In this study, a total of 370 faecal swab samples from 19 bat species were collected from various provinces of China and examined for the presence of AAVs. The mean prevalence rate was 22.4% (83 positives out of 370 samples), ranging from 10 to 38.9% among different bat species. The genome sequence spanning the entire rep-cap ORFs was determined from one chosen AAV-positive sample (designated BtAAV-YNM). Phylogenetic analysis of the entire rep-cap ORF coding sequences suggested that BtAAV-YNM is relatively distant to known primate AAVs, but phylogenetically closer to porcine AAV strain Po3. Further analysis of the partial cap ORF sequences of bat AAV samples (n=49) revealed a remarkably large genetic diversity, with an average pairwise nucleotide identity of only 84.3%. Co-presence of multiple distinctive genotypes of bat AAV within an individual sample was also observed. These results demonstrated that diverse AAVs might be widely distributed in bat populations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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China / epidemiology
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Chiroptera / virology*
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Cluster Analysis
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DNA, Viral / chemistry
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DNA, Viral / genetics
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Dependovirus / classification*
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Dependovirus / genetics
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Dependovirus / isolation & purification*
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Feces / virology
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Genetic Variation*
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Genotype
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Molecular Sequence Data
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Parvoviridae Infections / epidemiology
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Parvoviridae Infections / veterinary*
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Parvoviridae Infections / virology
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Phylogeny
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Prevalence
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Sequence Analysis, DNA
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Sequence Homology
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Viral Proteins / genetics
Substances
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DNA, Viral
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Viral Proteins
Associated data
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GENBANK/GU226879
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GENBANK/GU226880
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GENBANK/GU226881
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GENBANK/GU226882
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GENBANK/GU226883
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GENBANK/GU226884
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GENBANK/GU226885
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