Abstract
The photoreceptor and PAS/LOV protein VIVID (VVD) modulates blue-light signaling and influences light and temperature responses of the circadian clock in Neurospora crassa. One of the main actions of VVD on the circadian clock is to influence circadian clock phase by regulating levels of the transcripts encoded by the central clock gene frequency (frq). How this regulation is achieved is unknown. Here we show that VVD interacts with complexes central for circadian clock and blue-light signaling, namely the WHITE-COLLAR complex (WCC) and FREQUENCY-interacting RNA helicase (FRH), a component that complexes with FRQ to mediate negative feedback control in Neurospora. VVD interacts with FRH in the absence of WCC and FRQ but does not seem to control the exosome-mediated negative feedback loop. Instead, VVD acts to modulate the transcriptional activity of the WCC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Nucleus / metabolism
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Circadian Rhythm / genetics
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Circadian Rhythm / physiology*
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Cytoplasm / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Feedback, Physiological
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Fungal Proteins / genetics
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Fungal Proteins / physiology*
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Gene Expression Regulation, Fungal
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Genes, Fungal
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Light
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Models, Biological
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Neurospora crassa / genetics
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Neurospora crassa / physiology*
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Neurospora crassa / radiation effects*
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Photoreceptors, Microbial / genetics
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Photoreceptors, Microbial / physiology
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RNA Helicases / genetics
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RNA Helicases / physiology*
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RNA, Fungal / genetics
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RNA, Fungal / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Signal Transduction
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Transcription Factors / genetics
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Transcription Factors / physiology*
Substances
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DNA-Binding Proteins
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Fungal Proteins
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Photoreceptors, Microbial
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RNA, Fungal
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RNA, Messenger
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Transcription Factors
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VVD protein, Neurospora crassa
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wc-1 protein, Neurospora crassa
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white collar 2 protein, Neurospora
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RNA Helicases