Abstract
A newly identified virulent Cronobacter sakazakii phage, ESSI-2, was isolated from fecal samples from swine. The morphological characteristics evident under a transmission electron microscope indicated that phage ESSI-2 belonged to the family Myoviridae. The genome of phage ESSI-2 comprised a double-stranded DNA of 28,765 bp with a G+C content of 55.17%. Bioinformatic analysis of the phage genome identified 36 putative open reading frames (ORFs). The genome of phage ESSI-2 was not significantly similar to that of a previously reported bacteriophage of the members of Enterobacteriaceae. A lysogeny module was found within the genome of this virulent phage.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacteriophages / classification
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Bacteriophages / genetics*
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Bacteriophages / isolation & purification
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Bacteriophages / ultrastructure
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Base Composition
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DNA / genetics
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DNA, Viral / chemistry*
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DNA, Viral / genetics*
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Enterobacteriaceae / isolation & purification
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Enterobacteriaceae / virology*
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Feces / microbiology*
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Genome, Viral*
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Microscopy, Electron, Transmission
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Molecular Sequence Data
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Myoviridae / classification
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Myoviridae / genetics
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Myoviridae / isolation & purification
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Myoviridae / ultrastructure
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Open Reading Frames
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Sequence Analysis, DNA
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Sequence Homology
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Swine
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Virion / ultrastructure