Abstract
The glucose-regulated protein 78 (GRP78) is a key regulator of the endoplasmic reticulum (ER) stress response. This chapter discusses how GRP78 may be measured in vitro and in vivo. Because of the authors' expertise and experience with brain tumors, most of our work on GRP78 has focused on malignant gliomas. Here, we present our methodology for determining GRP78 in vitro using glioma cell lines and primary cell cultures, and in measuring GRP78 expression in tissue sections from rodent glioma models and human brain tumors. After discussion of measurement of GRP78 levels, modulation of GRP78 in vitro is demonstrated using glioma cell lines. Lastly, in vivo modulation of GRP78 levels via chemotherapy is determined using intracranial rodent models.
Copyright © 2011 Elsevier Inc. All rights reserved.
MeSH terms
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Activating Transcription Factor 6 / metabolism
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Animals
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Antineoplastic Agents / pharmacology
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Antineoplastic Agents / therapeutic use
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Brain Neoplasms / drug therapy*
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Brain Neoplasms / genetics
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Brain Neoplasms / metabolism
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Catechin / analogs & derivatives
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Catechin / pharmacology
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Catechin / therapeutic use
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Cell Line, Tumor
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Cells, Cultured
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Cytological Techniques*
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Dacarbazine / analogs & derivatives
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Dacarbazine / pharmacology
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Dacarbazine / therapeutic use
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Endoplasmic Reticulum Chaperone BiP
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Gene Expression Regulation, Neoplastic
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Glioma / drug therapy*
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Glioma / genetics
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Glioma / metabolism
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Heat-Shock Proteins / analysis
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / metabolism*
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Humans
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Immunohistochemistry / methods
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Mice
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Rats
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Temozolomide
Substances
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Activating Transcription Factor 6
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Antineoplastic Agents
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Endoplasmic Reticulum Chaperone BiP
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HSPA5 protein, human
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Heat-Shock Proteins
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Hspa5 protein, mouse
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Dacarbazine
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Catechin
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epigallocatechin gallate
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Temozolomide