Abstract
We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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Cytoplasm / metabolism
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Cytoplasm / virology
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Exons
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Genome, Viral
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HIV-1 / genetics*
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Human Immunodeficiency Virus Proteins / genetics
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Human Immunodeficiency Virus Proteins / metabolism
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Humans
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Nuclear Proteins / genetics*
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Phosphoproteins / genetics*
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Plasmids
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Proviruses / genetics
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RNA Splicing*
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RNA, Messenger
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RNA, Viral / genetics
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RNA, Viral / metabolism
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RNA-Binding Proteins
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Reverse Transcriptase Polymerase Chain Reaction
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Serine-Arginine Splicing Factors
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Viral Regulatory and Accessory Proteins / genetics
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Viral Regulatory and Accessory Proteins / metabolism
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env Gene Products, Human Immunodeficiency Virus / genetics
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env Gene Products, Human Immunodeficiency Virus / metabolism
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rev Gene Products, Human Immunodeficiency Virus / genetics
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rev Gene Products, Human Immunodeficiency Virus / metabolism
Substances
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Human Immunodeficiency Virus Proteins
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Nuclear Proteins
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Phosphoproteins
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RNA, Messenger
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RNA, Viral
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RNA-Binding Proteins
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SRSF6 protein, human
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Viral Regulatory and Accessory Proteins
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env Gene Products, Human Immunodeficiency Virus
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rev Gene Products, Human Immunodeficiency Virus
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vpu protein, Human immunodeficiency virus 1
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Serine-Arginine Splicing Factors