The aim of this study was to analyze the influence of individualized training (IT) as a function of motor type and effort status on changes of in specific physiological parameters among young soccer competitors. Blood pH and lactate concentrations, and lactate dehydrogenase (LDH), and creatine kinase (CK) activities were measured at the beginning of a preparation period, a match season, and a recuperation period of a 6-month macrocycle. The differences among specific physiological parameters as a function of the preparation phase for a defined motor type were analyzed by means of a 1-way generalized linear model (GLM) for repeated measurements. The differences in physiological parameters among defined motor types for a defined preparation phase were analyzed by means of the GLM for independent data. The differences in specific parameters before and after short time effort were analyzed by means of a t-test for matched pairs. Applied experimental and analytical approaches have revealed that IT administered to specific motor types differentiates players with respect to the pH, lactate concentration, and LDH activity. Obtained results indicate also that the dynamics of these parameters reflects the player's fitness level. Analysis of CK activity as a function of a preparation phase may serve as a prognostic tool for both overtraining and physical exhaustion.