Insertion sequence ISAba11 is involved in colistin resistance and loss of lipopolysaccharide in Acinetobacter baumannii

Jennifer H Moffatt; Marina Harper; Ben Adler; Roger L Nation; Jian Li; John D Boyce

doi:10.1128/AAC.01732-10

Insertion sequence ISAba11 is involved in colistin resistance and loss of lipopolysaccharide in Acinetobacter baumannii

Antimicrob Agents Chemother. 2011 Jun;55(6):3022-4. doi: 10.1128/AAC.01732-10. Epub 2011 Mar 14.

Authors

Jennifer H Moffatt¹, Marina Harper, Ben Adler, Roger L Nation, Jian Li, John D Boyce

Affiliation

¹ Department of Microbiology, Monash University, Victoria, Australia.

Abstract

Infections caused by Acinetobacter baumannii are of increasing concern, largely due to the multidrug resistance of many strains. Here we show that insertion sequence ISAba11 movement can result in inactivation of the A. baumannii lipid A biosynthesis genes lpxA and lpxC, resulting in the complete loss of lipopolysaccharide production and high-level colistin resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii / drug effects*
Acinetobacter baumannii / genetics
Acinetobacter baumannii / metabolism
Anti-Bacterial Agents / pharmacology*
Colistin / pharmacology*
DNA Transposable Elements*
Lipid A / biosynthesis
Lipopolysaccharides / metabolism*

Substances

Anti-Bacterial Agents
DNA Transposable Elements
Lipid A
Lipopolysaccharides
Colistin