Inhibitory effect of Pleurotus eryngii extracts on the activities of allergic mediators in antigen-stimulated mast cells

Eun Hee Han; Yong Pil Hwang; Hyung Gyun Kim; Jae Ho Choi; Ji Hye Im; Ji Hye Yang; Hyun-Uk Lee; Sung-Sik Chun; Young Chul Chung; Hye Gwang Jeong

doi:10.1016/j.fct.2011.03.034

Inhibitory effect of Pleurotus eryngii extracts on the activities of allergic mediators in antigen-stimulated mast cells

Food Chem Toxicol. 2011 Jun;49(6):1416-25. doi: 10.1016/j.fct.2011.03.034. Epub 2011 Mar 31.

Authors

Eun Hee Han¹, Yong Pil Hwang, Hyung Gyun Kim, Jae Ho Choi, Ji Hye Im, Ji Hye Yang, Hyun-Uk Lee, Sung-Sik Chun, Young Chul Chung, Hye Gwang Jeong

Affiliation

¹ Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.

PMID: 21440028
DOI: 10.1016/j.fct.2011.03.034

Abstract

Pleurotus eryngii is an edible mushroom native to Europe, the Middle East, and North Africa, and is also grown in parts of Asia. The present study investigated the anti-allergy potential of P. eryngii extract (PEE) in antigen-stimulated RBL-2H3 mast cells. PEE inhibited allergy markers, including release of hexosaminidase and histamine, in antigen-sensitized RBL-2H3 cells. PEE also suppressed the expression and production of interleukin-4 and reduced antigen-induced NFAT and NF-κB transcriptional activity in antigen-sensitized mast cells. Moreover, PEE decreased the levels of proinflammatory cytokines and COX-2 and iNOS expression in antigen-sensitized mast cells. Finally, PEE suppressed antigen-induced signal protein phosphorylation of Lyn, PLCγ2, PKC, Akt, and MAP kinases. Taken together, these results suggest that P. eryngii extract may provide insight into the prevention and treatment of allergic and inflammatory diseases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Allergic Agents / pharmacology*
Cell Line
Cell Proliferation / drug effects
Cyclooxygenase 2 / genetics
Cyclooxygenase 2 / metabolism
Cytokines / metabolism
Gene Expression / drug effects
Hexosaminidases / metabolism
Histamine Release / drug effects
Hypersensitivity / drug therapy*
Immunoglobulin E / immunology
Korea
Mast Cells / drug effects*
Mast Cells / immunology
Mast Cells / metabolism
Medicine, Traditional
NF-kappa B / genetics
NF-kappa B / metabolism
NFATC Transcription Factors / genetics
NFATC Transcription Factors / metabolism
Nitric Oxide Synthase Type II / genetics
Nitric Oxide Synthase Type II / metabolism
Plant Extracts / pharmacology*
Plants, Medicinal / chemistry
Pleurotus / chemistry*

Substances

Anti-Allergic Agents
Cytokines
NF-kappa B
NFATC Transcription Factors
Plant Extracts
Immunoglobulin E
Nitric Oxide Synthase Type II
Nos2 protein, rat
Cyclooxygenase 2
Ptgs2 protein, rat
Hexosaminidases