Eps8 regulates hair bundle length and functional maturation of mammalian auditory hair cells

PLoS Biol. 2011 Apr;9(4):e1001048. doi: 10.1371/journal.pbio.1001048. Epub 2011 Apr 19.

Abstract

Hair cells of the mammalian cochlea are specialized for the dynamic coding of sound stimuli. The transduction of sound waves into electrical signals depends upon mechanosensitive hair bundles that project from the cell's apical surface. Each stereocilium within a hair bundle is composed of uniformly polarized and tightly packed actin filaments. Several stereociliary proteins have been shown to be associated with hair bundle development and function and are known to cause deafness in mice and humans when mutated. The growth of the stereociliar actin core is dynamically regulated at the actin filament barbed ends in the stereociliary tip. We show that Eps8, a protein with actin binding, bundling, and barbed-end capping activities in other systems, is a novel component of the hair bundle. Eps8 is localized predominantly at the tip of the stereocilia and is essential for their normal elongation and function. Moreover, we have found that Eps8 knockout mice are profoundly deaf and that IHCs, but not OHCs, fail to mature into fully functional sensory receptors. We propose that Eps8 directly regulates stereocilia growth in hair cells and also plays a crucial role in the physiological maturation of mammalian cochlear IHCs. Together, our results indicate that Eps8 is critical in coordinating the development and functionality of mammalian auditory hair cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acoustic Stimulation
  • Action Potentials
  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Animals
  • Calcium / metabolism
  • Calcium Channels / metabolism
  • Cell Surface Extensions / metabolism*
  • Cell Surface Extensions / ultrastructure
  • Cochlea / cytology
  • Cochlea / growth & development
  • Cochlea / physiology*
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / metabolism*
  • Deafness / genetics
  • Evoked Potentials, Auditory, Brain Stem
  • Exocytosis
  • Gene Deletion
  • Hair Cells, Auditory / metabolism*
  • Hair Cells, Auditory / ultrastructure
  • Mechanotransduction, Cellular
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Potassium Channels / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • Calcium Channels
  • Cytoskeletal Proteins
  • Eps8 protein, mouse
  • Potassium Channels
  • Calcium