Multi-day pre-clinical demonstration of glucose/galactose binding protein-based fiber optic sensor

Biosens Bioelectron. 2011 Jun 15;26(10):4117-23. doi: 10.1016/j.bios.2011.04.007. Epub 2011 Apr 13.

Abstract

We report here the first pre-clinical demonstration of continuous glucose tracking by fluorophore-labeled and genetically engineered glucose/galactose binding protein (GGBP). Acrylodan-labeled GGBP was immobilized in a hydrogel matrix at the tip of a small diameter optical fiber contained in a stainless steel needle. The fiber optic biosensors were inserted subcutaneously into Yucatan and Yorkshire swine, and the sensor response to changing glucose levels was monitored at intervals over a 7-day period. Sensor mean percent error on day 7 was 16.4±5.0% using a single daily reference blood glucose value to calibrate the sensor. The GGBP sensor's susceptibility to common interferents was tested in a well-plate system using human sera. No significant interference was observed from the tested interferents except for tetracycline at the drug's maximum plasma concentration. The robust performance of the GGBP-based fiber optic sensor in swine models and resistance to interferents indicates the potential of this technology for continuous glucose monitoring in humans.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Blood Glucose / analysis*
  • Escherichia coli Proteins* / chemistry
  • Humans
  • Immobilized Proteins / chemistry
  • Models, Animal
  • Monitoring, Physiologic / instrumentation
  • Monitoring, Physiologic / methods
  • Monosaccharide Transport Proteins* / chemistry
  • Optical Fibers
  • Protein Conformation
  • Sus scrofa
  • Swine
  • Swine, Miniature

Substances

  • Blood Glucose
  • Escherichia coli Proteins
  • Immobilized Proteins
  • Monosaccharide Transport Proteins
  • mglB protein, E coli