Abstract
The tat-responsive region (TAR) of the human immunodeficiency virus-1 (HIV-1) exhibits a trans-inhibitory effect on translation in vitro by activating the interferon-induced 68-kilodalton protein kinase (p68 kinase). Productive infection by HIV-1 was shown to result in a significant decrease in the amount of cellular p68 kinase. The steady-state amount of p68 kinase was also reduced in interferon-treated HeLa cell lines stably expressing tat, as compared to the amount of the kinase in interferon-treated control HeLa cells. Thus, the potential translational inhibitory effects of the TAR RNA region mediated by activation of p68 kinase may be downregulated by tat during productive HIV-1 infection.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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2',5'-Oligoadenylate Synthetase / genetics
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Down-Regulation
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Enzyme Induction
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Gene Expression Regulation, Enzymologic*
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Gene Products, tat / physiology*
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Genes, Viral*
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Genes, tat*
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HIV-1 / genetics*
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HeLa Cells
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Humans
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Immunosorbent Techniques
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Interferon Type I / pharmacology*
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Molecular Weight
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Protein Kinases / biosynthesis
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Protein Kinases / genetics*
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Trans-Activators / physiology*
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Transfection
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tat Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, tat
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Interferon Type I
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Trans-Activators
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tat Gene Products, Human Immunodeficiency Virus
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Protein Kinases
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2',5'-Oligoadenylate Synthetase