Abstract
Objective:
To explore the mechanism of nourishing kidney and reducing urine effect of suoquan capsule by discussing the adjusting action on ALD synthase.
Methods:
Built the model of deficiency of the kidney and diuresis by adenine,inspectd the contents of Cort, ALD in blood and the mRNA expression of CYP11B2 with ELISA and RT-PCR technology of the mice.
Results:
Compared with model group, Suoquan capsule could remarkedly increase the contents of Cort, ALD in blood and the mRNA expression of CYP1 1 B2 of model rats.
Conclusions:
Suoquan capsule can increase the contents of Cort, ALD in blood and the mRNA expression of CYP11B2 in deficiency of the kidney and diuresis rats. Promote the combining of ALD by adjusting the ALD synthase may be the mechanism of nourishing the kidney and reducing urine of Souquan capsule from ALD synthase approach.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenine / adverse effects
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Aldosterone / blood
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Animals
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Corticosterone / blood
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Cytochrome P-450 CYP11B2 / genetics
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Cytochrome P-450 CYP11B2 / metabolism*
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Disease Models, Animal
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Diuretics / pharmacology
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Diuretics / therapeutic use
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Drug Combinations
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Drugs, Chinese Herbal / administration & dosage
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Drugs, Chinese Herbal / pharmacology*
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Drugs, Chinese Herbal / therapeutic use
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Female
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Kidney / drug effects
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Kidney / metabolism*
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Kidney Diseases / chemically induced
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Kidney Diseases / drug therapy*
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Kidney Diseases / metabolism
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Plants, Medicinal / chemistry
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Diuretics
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Drug Combinations
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Drugs, Chinese Herbal
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RNA, Messenger
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Aldosterone
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Cytochrome P-450 CYP11B2
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Adenine
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Corticosterone