Abstract
We show that TR4 facilitates lipid accumulation in 3T3-L1 adipocytes via induction of the FATP1 gene. Further study showed that TR4 transactivated FATP1 5' promoter activity via direct binding to the TR4 responsive element located at the FATP1 5' promoter region. Constitutive overexpression of TR4 in 3T3-L1 adipocytes resulted in increased lipid accumulation, accompanied by an increase in fatty acid uptake. However, small interfering RNA knockdown of FATP1 abolished TR4-enhanced fatty acid uptake. Moreover, microRNA-mediated silencing of TR4 in 3T3-L1 adipocytes drastically reduced basal FATP1 5' promoter activity and FATP1 expression with reduced lipid accumulation.
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3-L1 Cells
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Adipocytes / metabolism*
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Animals
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Blotting, Western
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CD36 Antigens / genetics
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CD36 Antigens / metabolism
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Chromatin Immunoprecipitation
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Coenzyme A Ligases / genetics
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Coenzyme A Ligases / metabolism
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Fatty Acid Transport Proteins / genetics
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Fatty Acid Transport Proteins / metabolism*
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Fatty Acid-Binding Proteins / genetics
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Fatty Acid-Binding Proteins / metabolism
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Fatty Acids / metabolism*
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Mice
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MicroRNAs / genetics
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Nuclear Receptor Subfamily 2, Group C, Member 2 / genetics
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Nuclear Receptor Subfamily 2, Group C, Member 2 / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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CD36 Antigens
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Fabp4 protein, mouse
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Fatty Acid Transport Proteins
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Fatty Acid-Binding Proteins
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Fatty Acids
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MicroRNAs
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Nuclear Receptor Subfamily 2, Group C, Member 2
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Slc27a1 protein, mouse
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Slc27a4 protein, mouse
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ACSL1 protein, mouse
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Coenzyme A Ligases