Analysis of a next-generation sequence dataset from Mirabilis jalapa resulted in the discovery of a novel virus in the genus Carlavirus (family Betaflexiviridae), mirabilis jalapa mottle virus (MjMV). The complete genome of MjMV was determined to consist of 8315 nucleotides (nt), with the six open reading frames indicative of carlaviruses. MjMV is most similar to kalanchoe latent virus (60% identity) and lily symptomless virus (59% identity). The virus can be transmitted mechanically to Mirabilis, but thus far MjMV has only been shown to infect Mirabilis jalapa, causing a slight leaf mottling and leaf wrinkling phenotype.